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Junior Specialist / Assistant Specialist - Chemical and Biomolecular Engineering - College of Chemistry
Position overview
salary scale(s) for this position: Specialist Salary Scales. A reasonable full time salary estimate for this position is $57,000 - $65,800.
Application Window
Open date: July 29, 2026
Next review date: Wednesday, Aug 12, 2026 at 11:59pm (Pacific Time)
Apply by this date to ensure full consideration by the committee.
Final date: Sunday, Aug 30, 2026 at 11:59pm (Pacific Time)
Applications will continue to be accepted until this date, but those received after the review date will only be considered if the position has not yet been filled.
Position description
The employee will join the Shiyu Xia Lab at the Department of Chemical and Biomolecular Engineering, College of Chemistry, University of California, Berkeley and participate in research projects focused on molecular cloning, protein expression in bacterial and mammalian cells, protein purification using affinity chromatography and size exclusion chromatography, protein structural determination using electron microscopy and X-ray crystallography and determining the molecular structures of proteins to understand their biological mechanisms,
Duties will include:
- Conduct molecular cloning, plasmid construction, and DNA assembly using PCR, Gibson assembly, restriction enzyme cloning, bacterial transformation, and sequence verification.
- Perform recombinant protein expression in Escherichia coli and mammalian cell lines through bacterial culture, transient transfection, and stable cell line generation.
- Purify proteins using affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography (FPLC), followed by biochemical characterization using SDS-PAGE, Western blotting, and analytical chromatography.
- Perform structural biology experiments, including protein crystallization, X-ray crystallography, negative-stain electron microscopy, cryo-electron microscopy sample preparation, image acquisition, and structural data analysis.
- Conduct mammalian cell culture, fluorescence microscopy, flow cytometry, and quantitative cellular assays to investigate protein function and signaling pathways.
- Design, execute, and analyze biochemical and biophysical assays to characterize protein-protein interactions, enzyme activity, and biomolecular assemblies.
- Analyze experimental data using appropriate computational and statistical methods, maintain detailed laboratory records, assist with laboratory organization and safety compliance, and contribute to manuscript preparation and presentation of research findings.
Qualifications
Bachelor's degree (or equivalent international degree) or enrolled in a Bachelor's degree (or equivalent international degree) program
Bachelor's degree (or equivalent international degree)
- Prior research experience in one or more of the following areas: molecular biology, biochemistry, cell biology, protein engineering, synthetic biology, or bioengineering.
- Experience with standard laboratory techniques, including molecular cloning, protein purification, mammalian cell culture, microscopy, or related experimental methods.
- Strong organizational skills, attention to detail, and ability to work both independently and collaboratively in a research environment.
- Excellent written and verbal communication skills.
Application Requirements
Curriculum Vitae - Your most recently updated C.V.
Cover Letter
- 2 required (contact information only)
Help contact: hsangera1@berkeley.edu
About UC Berkeley
UC Berkeley is committed to diversity, equity, inclusion, and belonging in our public mission of research, teaching, and service, consistent with UC Regents Policy 4400 and University of California Academic Personnel policy (APM 210 1-d). These values are embedded in our Principles of Community, which reflect our passion for critical inquiry, debate, discovery and innovation, and our deep commitment to contributing to a better world. Every member of the UC Berkeley community has a role in sustaining a safe, caring and humane environment in which these values can thrive.
The University of California, Berkeley is an Equal Opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, or protected veteran status.
For more information, please refer to the University of California’s Affirmative Action and Nondiscrimination in Employment Policy and the University of California’s Anti-Discrimination Policy.
In searches when letters of reference are required all letters will be treated as confidential per University of California policy and California state law. Please refer potential referees, including when letters are provided via a third party (i.e., dossier service or career center), to the UC Berkeley statement of confidentiality prior to submitting their letter.
As a University employee, you will be required to comply with all applicable University policies and/or collective bargaining agreements, as may be amended from time to time. Federal, state, or local government directives may impose additional requirements.
Unless stated otherwise, unambiguously, in the position description, this position does not include sponsorship of a new consular H-1B visa petition that would require payment of the $100,000 supplemental fee.
As a condition of employment, the finalist will be required to disclose if they are subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct.
- “Misconduct” means any violation of the policies or laws governing conduct at the applicant’s previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment or discrimination, as defined by the employer.
- UC Sexual Violence and Sexual Harassment Policy
- UC Anti-Discrimination Policy
- APM - 035: Affirmative Action and Nondiscrimination in Employment